Improving administration regimens of CyaA-based vaccines using TRAP assays to detect antigen-specific CD8(+) T cells directly ex vivo.
Vaccine
; 27(41): 5565-73, 2009 Sep 18.
Article
em En
| MEDLINE
| ID: mdl-19647811
ABSTRACT
Vaccination with recombinant adenylate cyclase of Bordetella pertussis (CyaA) carrying antigen is a promising approach to target antigen-presenting cells. We have used Trogocytosis Analysis Protocol (TRAP) assays to monitor immune responses raised by different vaccination regimens with recombinant CyaA carrying the ovalbumin antigen. We find that the intradermal, intramuscular or subcutaneous routes are all superior to intravenous injections, and actually lead to a sufficiently high frequency of reactive CTL to be detected and characterized directly ex vivo by TRAP assay or other standard assays. Finally, for all routes, we find a clear boosting effect upon re-injection of the vaccine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T Citotóxicos
/
Ovalbumina
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Linfócitos T CD8-Positivos
/
Toxina Adenilato Ciclase
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
França