Peripheral Thy1+ lymphocytes rearranging TCR-gammadelta genes in LAT-deficient mice.
Eur J Immunol
; 39(9): 2596-605, 2009 Sep.
Article
em En
| MEDLINE
| ID: mdl-19701892
Linker for activation of T cells (LAT) is an adaptor molecule indispensable for development of alphabeta and gammadelta T lymphocytes. Surprisingly, using a new model of LAT-deficient mice we found that despite arrested thymic development, a discrete population of cells with active Lat promoter, expressing Thy1 molecules, accumulated in peripheral lymphoid organs of homozygous (Lat(Inv/Inv)) mutant mice. By measuring frequencies of TCR gene rearrangements in conjunction with a panel of cell surface Ag, we dissected two subsets of these Thy1(+) cells. Thy1(dull) cells expressed markers of NK lymphocytes and contained low frequency of TCR-gamma gene rearrangements without detectable TCR-delta rearrangements. Thy1(high) cells resembled immature CD44(+)CD25(+) thymocytes and contained high frequency of non-productive TCR-gamma and TCR-delta rearrangements, indicating that cells displaying molecular signatures of commitment toward gammadelta T-cell lineage can develop and populate lymphoid tissues of LAT-deficient mice. Phenotypically similar Thy1(high) cells were also found in lymph nodes of lymphocyte-deficient (Rag2(-/-)) mice but not in T lymphocyte proficient, heterozygous Lat(+/Inv) mice suggesting that Thy1(high) cells of LAT-deficient mice identified in this study accumulate in peripheral lymphoid organs as a result of congenital lymphopenia.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
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Linfócitos T
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Receptores de Antígenos de Linfócitos T gama-delta
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Rearranjo Gênico da Cadeia delta dos Receptores de Antígenos dos Linfócitos T
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Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T
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Antígenos Thy-1
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Proteínas Adaptadoras de Transdução de Sinal
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Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Polônia