[Preparation and identification of polyclonal antibody against protein H1b: the variant of major subunit of human ASGPR].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 25(10): 917-9, 2009 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-19811742
AIM: To prepare and identify mouse polyclonal antibody against protein H1b, which is the variant of major subunit of human ASGPR. METHODS: H1b specific peptide was synthesized and coupled with keyhole limpet hemocyanin (KLH) for immunization. Then H1b-KLH conjugation was injected into mouse subcutaneously to produce polyclonal antibody. ELISA assay was used to detect the titer of the antibody. Antibody was also identified by Western blot and immunohistochemistry assays. RESULTS: Mouse antibody against H1b was prepared after injection of H1b-KLH conjugation. The titer of H1b antibody was about 1:10(5). Western blot confirmed its high specificity. This antibody could also be used for immunohistochemistry analysis. CONCLUSION: The successful preparation of the polyclonal antibody against protein H1b, which can discriminate the two variants of the major subunit of ASGPR with high specificity, will provide an efficient reagent for further study of the physiologic functions of H1b and its role in the pathogenesis of human disease.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptor de Asialoglicoproteína
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China