[Amelogenin expression in cultured human dental pulp and periodontium related cells].
Shanghai Kou Qiang Yi Xue
; 18(5): 499-504, 2009 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-19907857
ABSTRACT
PURPOSE:
To investigate gene expression of amelogenin (Am) in human gingival epithelial cells(HGEC) and also other oral ectomesenchyme cells (human gingival fibroblasts, human periodontal ligament fibroblasts and human pulp cells).METHODS:
The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.RESULTS:
There was no amelogenin expression detected in any of the cells.CONCLUSIONS:
These findings suggest that amelogenin expression could not be detected in cultured human periodontium-related cells. Supported by National Natural Science Foundation of China (Grant No.30672315) and Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polpa Dentária
/
Amelogenina
Limite:
Humans
Idioma:
Zh
Revista:
Shanghai Kou Qiang Yi Xue
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China