Variability of DNA microarray gene expression profiles in cultured rat primary hepatocytes.
Gene Regul Syst Bio
; 1: 235-49, 2007 Nov 18.
Article
em En
| MEDLINE
| ID: mdl-19936092
ABSTRACT
DNA microarray is a powerful tool in biomedical research. However, transcriptomic profiling using DNA microarray is subject to many variations including biological variability. To evaluate the different sources of variation in mRNA gene expression profiles, gene expression profiles were monitored using the Affymetrix RatTox U34 arrays in cultured primary hepatocytes derived from six rats over a 26 hour period at 6 time points (0 h, 2h, 5h, 8h, 14 h and 26 h) with two replicate arrays at each time point for each animal. In addition, the impact of sample size on the variability of differentially expressed gene lists and the consistency of biological responses were also investigated. Excellent intra-animal reproducibility was obtained at all time points with 0 out of 370 present probe sets across all time points showing significant difference between the 2 replicate arrays (3-way ANOVA, p
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Gene Regul Syst Bio
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos