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Oxidative stress promotes autophagic cell death in human neuroblastoma cells with ectopic transfer of mitochondrial PPP2R2B (Bbeta2).
Cheng, Wan-Ting; Guo, Zhi-Xuan; Lin, Chia-An; Lin, Ming-Yi; Tung, Li-Chu; Fang, Kang.
Afiliação
  • Cheng WT; Department of Life Science, National Taiwan Normal University, Taipei, Taiwan. srnt.tw@yahoo.com.tw
BMC Cell Biol ; 10: 91, 2009 Dec 18.
Article em En | MEDLINE | ID: mdl-20017961
ABSTRACT

BACKGROUND:

The multifunctional protein phosphatase 2A (PP2A) is a heterotrimeric serine/threonine protein phosphatase composed of a scaffolding, catalytic and regulatory subunits. By modifying various downstream signal transducers, the aberrant expression of the brain-targeted regulatory subunit PPP2R2B is associated with the onset of a panel of neuronal disorders. The alternatively splicing of PPP2R2B encodes two regulatory subunit isoforms that determine cellular distribution of the neuron-specific holoenzyme to mitochondria (Bbeta2) and cytoplasm (Bbeta1), respectively.

RESULTS:

Human neuroblastoma cells were transfected with PPP2R2B constructs encoding the complete sequences of Bbeta2 and Bbeta1, respectively. The colonies with antibiotic resistance were selected as stable cell lines. Both ectopic Bbeta1 and Bbeta2 clones exhibited characteristics of autophagy. To test how cells respond to reactive oxygen species generators, the cells were treated with either hydrogen peroxide or t-butyl hydroperoxide and Bbeta2 clones induced cell death. Suppression of autophagy using either RNA interference of the essential autophagy gene or pharmacological inhibitor rescued cell death caused by oxidative stress.

CONCLUSIONS:

Cells with ectopically expressed mitochondria-targeted regulatory subunit PPP2R2B of the holoenzyme PP2A were shown predisposed to autophagy and oxidative stress induced cell death that is related to apoptosis. The results promised a model for studying the mechanism and function of aberrant PPP2R2B expression in neuronal cells. The work provided a new target for understanding and prevention of neuropathogenesis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Estresse Oxidativo / Proteína Fosfatase 2 / Mitocôndrias / Proteínas do Tecido Nervoso / Neuroblastoma Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: BMC Cell Biol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Taiwan

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Estresse Oxidativo / Proteína Fosfatase 2 / Mitocôndrias / Proteínas do Tecido Nervoso / Neuroblastoma Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: BMC Cell Biol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Taiwan