The differentiation and engraftment potential of mouse hematopoietic stem cells is maintained after bio-electrospray.
Analyst
; 135(1): 157-64, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-20024196
ABSTRACT
The bio-electrospray technique has been recently pioneered to manipulate living, immortalised and primary cells, including a wide range of stem cells. Studies have demonstrated that the creation of viable, fully functional in vitro microenvironments is possible using this technique. By modifying the bio-electrospray procedure (referred to as cell electrospinning), a variety of microenvironment morphologies have been fabricated. Because bio-electrospraying of biological material is a relatively new technique, it is important to determine if there are any unwanted consequences to the manipulated cells as a result of the procedure. Here, we establish the validity of the process using a heterogeneous, living population of hematopoietic stem/progenitor cells, using a functional in vitro assay and in vivo mouse model to investigate for side-effects that previous in vitro assays may not have detected. Our studies demonstrate that these bio-protocols have no obvious negative effects, thus indicating significant promise for utility in biological sciences and for a plethora of healthcare applications.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Hematopoéticas
/
Separação Celular
Limite:
Animals
Idioma:
En
Revista:
Analyst
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido