Simplified method for isolation of white cells from whole blood suitable for direct polymerase chain reaction.
Biotechniques
; 10(1): 39-40, 1991 Jan.
Article
em En
| MEDLINE
| ID: mdl-2003919
ABSTRACT
A simple method for isolating mononuclear cells from whole blood is described. The procedure utilizes phytohemagglutinin to agglutinate the erythrocytes, separating white cells from whole blood in a very brief handling time. The isolated cells are readily subjected to DNA isolation simply by boiling, and the released DNA can be directly employed for the polymerase chain reaction analysis. The efficiency of this method is similar to other conventional methods, but less costly and less time-consuming. This method is particularly useful in analyzing DNA samples from the peripheral blood cells when the simplicity and low cost of the assay are preferable.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
/
Separação Celular
/
Reação em Cadeia da Polimerase
Limite:
Humans
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1991
Tipo de documento:
Article