A novel enzyme-linked immunosorbent assay (ELISA) for the detection of beryllium antibodies.
J Immunol Methods
; 137(1): 65-72, 1991 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-2010619
ABSTRACT
A novel immunological method has been developed for detecting antibodies (IgG molecules) specific to beryllium, a light metal used in industry and capable of causing chronic beryllium disease. Beryllium metal was vacuum deposited onto commercially available immunological microsticks, which were then exposed to test plasma containing the putative antibodies. Antigen-antibody complexes were located using a biotin-avidin amplification method. One employee diagnosed with chronic beryllium disease and one diagnosed as "sensitized" (lymphocyte transformation positive) exhibited antibody titers graphically and statistically different and higher than a pooled baseline control population. Plasma from these two employees (former beryllium workers) was used in four different approaches to validate the presence of beryllium antibodies. The assay proved to be reproducible.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Berílio
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1991
Tipo de documento:
Article