Modification of the stability of phospholipase A2 by charge engineering.
FEBS Lett
; 281(1-2): 219-22, 1991 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-2015898
ABSTRACT
Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipases A
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido