Resistant starch, fermented resistant starch, and short-chain fatty acids reduce intestinal fat deposition in Caenorhabditis elegans.
J Agric Food Chem
; 58(8): 4744-8, 2010 Apr 28.
Article
em En
| MEDLINE
| ID: mdl-20353151
ABSTRACT
Obesity is a growing global public health dilemma. The objective of this project is to develop and validate a screening mechanism for bioactive compounds that may reduce body fat and promote health. Resistant starch (RS) reduces body fat in rodents. Amylose starch that has a high content of RS, endogenous compounds obtained from the ceca of amylose starch fed mice (fermented RS), and individual short-chain fatty acids (SCFA) were tested. The Caenorhabditis elegans model and Nile red staining were selected to determine the intestinal fat deposition response to bioactive components. The fluorescence intensity of Nile red was reduced to 76.5% (amylose starch), 78.8% (fermented RS), 63.6% (butyrate), or 28-80% (SCFAs) of controls, respectively (P < 0.001). The reduced intestinal fat deposition suggests reduced food intake or increased energy expenditure. C. elegans is a practical animal model to screen for bioactive compounds that may prevent or treat obesity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Amido
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Caenorhabditis elegans
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Gorduras
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Ácidos Graxos
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Fermentação
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Mucosa Intestinal
Limite:
Animals
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos