Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.
Graefes Arch Clin Exp Ophthalmol
; 229(2): 157-63, 1991.
Article
em En
| MEDLINE
| ID: mdl-2044978
ABSTRACT
Using the immunogold technique combined with cryoultramicrotomy and London Resin white (LR white) embedding, we studied the fine structural distribution of types I-IV collagen and laminin in corneal tissue from seven enucleated human eyes (age range, 63-78 years). Type II collagen was not identified in any corneal layer. Type I and type III collagen were distributed in a similar fashion in striated collagen fibrils in Bowman's layer and in the stroma. Type IV collagen was located only in the posterior non-banded region of Descemet's membrane. Laminin was identified in subepithelial anchoring plaques and the sub-endothelial region of Descemet's membrane in accordance with its recognized adhesive function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Matriz Extracelular
/
Colágeno
/
Laminina
/
Córnea
Limite:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Graefes Arch Clin Exp Ophthalmol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido