Visible fluorescence detection of type III protein secretion from bacterial pathogens.
J Am Chem Soc
; 132(24): 8244-5, 2010 Jun 23.
Article
em En
| MEDLINE
| ID: mdl-20504019
ABSTRACT
Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Proteínas de Bactérias
/
Salmonella enterica
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos