[Study on fingerprints spectrum of Lophatherum gracile by HPLC].

ABSTRACT

OBJECTIVE: To establish the fingerprint spectrum of Lophatherum gracile by HPLC. METHODS: The samples were separated on Agilent Eclipse XDB- C18 column (150 mm x 4.6 mm, 5 microm) with methanol (A) -3% acetic acid (B) as the mobile phase at a flow rate of 1.0 mL/min at 30 centigrades, adopting the gradient elution process with switching the Wavelength. RESULTS: 15 samples of different origin Lophatherum gracile Brongn were detected and 16 peaks in the chromatogram were common, there was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine. CONCLUSION: The method is accurate, reliable and the fingerprint spectrum provides more information for controlling the quality of Lophatherum gracile.