Dynamic coating of SU-8 microfluidic chips with phospholipid disks.
Electrophoresis
; 31(15): 2566-74, 2010 Aug.
Article
em En
| MEDLINE
| ID: mdl-20603829
ABSTRACT
In this work, PEG-stabilized phosphatidylcholine lipid aggregates (disks), mimicking mammalian cell membranes, were introduced as a new biofouling resistant coating for SU-8 polymer microchannels. A rapid and simple method was developed for immobilization of PEGylated phosphatidylcholine disks in microchannels. Microfluidic chips made from SU-8, PDMS, or glass were dynamically coated with the PEGylated disks followed by characterization of their surface chemistry before and after coating. On the basis of the observed changes in EOF and nonspecific protein adsorption, the affinity of the PEGylated disks was shown to be particularly strong toward SU-8. The PEG-lipid coating enabled permanent change in EOF in SU-8 microchannels with an initial value of 4.5 x 10(-8) m(2) V(-1) s(-1), decreasing to 2.1 x 10(-8) m(2) V(-1) s(-1) (immediately after modification), and, eventually, to 1.5 x 10(-8) m(2) V(-1) s(-1) (7 days after modification) for 9 mM sodium borate (pH 10.5) as BGE. As determined by the Wilhelmy plate measurements and microchip-CE analysis of BSA, the PEG-lipid coating also enabled efficient biofouling shield against protein adsorption, similar to that of low amounts of SDS (3.5 mM) or Tween-20 (80 microM) as buffer additives. These results suggest that dynamically attached PEG-lipid aggregates provide stable, biomimicking surface modification that efficiently reduces biofouling on SU-8.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Polietilenoglicóis
/
Técnicas Analíticas Microfluídicas
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Finlândia