Evaluation of human intestinal epithelial differentiated cells (Caco-2) for replication, plaque formation and isolation of avian influenza viruses.
J Virol Methods
; 169(1): 232-8, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-20674612
ABSTRACT
Although various cultured cells are used for propagating influenza A viruses, the types of cells which can support replication of and plaque production by low pathogenic avian influenza (LPAI) viruses without supplementary trypsin are limited. In this study, the infectivity and growth kinetics of as well as plaque production by LPAI viruses in Caco-2 cells were investigated. The suitability of this cell line for virus isolation was examined and compared with virus isolation in embryonated chicken eggs. Generation of Caco-2 mediated viral variants, if any, was assessed phenotypically and genotypically. It was found that Caco-2 cells can readily support continued replication of LPAI viruses without supplementary trypsin. Viruses replicate to high titer compared to embryonated chicken eggs, and more efficiently than in MDCK cells, without trypsin. Also, LPAI viruses produced plaques in Caco-2 cells. However, these cells were found to be less sensitive than embryonated chicken eggs for virus isolation. Notably, no phenotypic and genotypic changes of the viruses were observed during viral passages (at least up to 10th passage) in Caco-2 cells. These findings indicate that Caco-2 cells may provide an appropriate substrate for studying and cultivating AIVs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Ensaio de Placa Viral
/
Influenza Aviária
Tipo de estudo:
Evaluation_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão