Measuring the effect of ligand binding on the interface stability of multimeric proteins using dynamic light scattering.
Anal Biochem
; 407(2): 278-80, 2010 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-20732291
ABSTRACT
We have demonstrated that an approach using guanidine hydrochloride at low concentrations to progressively disrupt protein-protein interactions can be quantitated using dynamic light scattering. This approach is sensitive enough to detect ligand-induced changes of subunit-subunit interactions for homo-hexameric glutamate dehydrogenase, allowing ΔΔG of reversible subunit dissociation to be calculated. The use of dynamic light scattering makes this approach generally applicable to soluble proteins to monitor the relative strength of protein-protein interactions with a particular emphasis on assessing the impact of ligand binding on such interfaces.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espalhamento de Radiação
/
Glutamato Desidrogenase
/
Luz
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos