The preparation of certified calibration solutions for azaspiracid-1, -2, and -3, potent marine biotoxins found in shellfish.
Anal Bioanal Chem
; 398(5): 2243-52, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-20827466
ABSTRACT
The production and certification of a series of azaspiracid (AZA) calibration solution reference materials is described. Azaspiracids were isolated from contaminated mussels, purified by preparative liquid chromatography and dried under vacuum to the anhydrous form. The purity was assessed by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The final concentration of each AZA in a CD(3)OH stock solution was determined by quantitative NMR spectroscopy. This solution was then diluted very accurately in degassed, high purity methanol to a concentration of 1.47 ± 0.08 µmol/L for CRM-AZA1, 1.52 ± 0.05 µmol/L for CRM-AZA2, and 1.37 ± 0.13 µmol/L for CRM-AZA3. Aliquots were dispensed into argon-filled glass ampoules, which were immediately flame-sealed. The calibration solutions are suitable for method development, method validation, calibration of liquid chromatography or mass spectrometry instrumentation and quality control of shellfish monitoring programs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piranos
/
Frutos do Mar
/
Compostos de Espiro
/
Furanos
/
Toxinas Marinhas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Canadá