Assessing covalent binding of reactive drug metabolites by complete protein digestion and LC-MS analysis.
Bioanalysis
; 2(7): 1211-21, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-21083235
ABSTRACT
BACKGROUND:
Covalent binding by reactive drug metabolites represents a poorly understood cause of drug toxicity. Currently, assessing protein covalent binding usually entails the use of radioactive drug and therefore has limited applicability in drug discovery. Several marketed drugs are known to form reactive metabolites and have been shown to covalently bind to proteins.RESULTS:
In this article, we describe a new method for the analysis of reactive metabolite-protein binding by MS using a strategy of complete digestion of microsomal proteins into free amino acids. Immobilized pronase was found to be the best method for complete digestion in terms of stability of amino acid modifications as well as minimized spectral background.CONCLUSION:
Modified cysteine residues were identified for four tested drug compounds known to form reactive metabolites following in vitro microsomal incubations and accurate mass measurements by LC-MS analysis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Preparações Farmacêuticas
/
Proteínas
/
Cromatografia Líquida
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Canadá