Differences in innate immune function between allergic and nonallergic children: new insights into immune ontogeny.
J Allergy Clin Immunol
; 127(2): 470-478.e1, 2011 Feb.
Article
em En
| MEDLINE
| ID: mdl-21093030
ABSTRACT
BACKGROUND:
Microbial products are of central interest in the modulation of allergic propensity.OBJECTIVE:
We sought to explore whether allergic children show differences in microbial Toll-like receptor (TLR)-mediated responses over their first 5 years of life.METHODS:
Mononuclear cells isolated from 35 allergic and 35 nonallergic children at birth and 1, 2.5, and 5 years of age were stimulated with TLR2-TLR9 ligands to study innate immune function and with allergens or mitogen to assess adaptive T-cell responses. Cytokine production was measured by using Luminex multiplexing technology.RESULTS:
Nonallergic children show progressive and significant age-related increases in innate cytokine responses (IL-1ß, IL-6, TNF-α, and IL-10) to virtually all TLR ligands. This innate maturation corresponds with a parallel increase in adaptive T(H)1 (IFN-γ) responses to allergens and mitogens. In contrast, allergic children show exaggerated innate responses at birth (P < .01) but a relative decrease with age thereafter, so that by age 5 years, TLR responses are attenuated compared with those seen in nonallergic subjects (P < .05). This early hyperresponsiveness in allergic subjects fails to translate to a corresponding maturation of T(H)1 function, which remains attenuated relative to that seen in nonallergic subjects but is associated with a characteristic age-dependent increase in allergen-specific T(H)2 responses (P < .01).CONCLUSION:
Our findings suggest significant differences in the developmental trajectory of innate immune function in children with allergic disease that might contribute to the recognized differences in postnatal adaptive T-cell immunity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hipersensibilidade
/
Imunidade Inata
Tipo de estudo:
Observational_studies
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Risk_factors_studies
Limite:
Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Idioma:
En
Revista:
J Allergy Clin Immunol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Austrália