[Clinical and laboratory study of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRß abnormalities].
Zhonghua Xue Ye Xue Za Zhi
; 31(8): 540-4, 2010 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-21122334
ABSTRACT
OBJECTIVE:
To explore the clinical and laboratory characteristics of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRß abnormalities.METHODS:
Chromosome specimens were prepared directly and/or short-time culture of bone marrow cells. Karyotyping was performed with R-binding technique. Fluorescence in situ hybridization (FISH) was performed using PDGFRß, PDGFRα, FGFR1 break-apart probes and whole chromosome 5 and 12 painting probes, respectively. The expression of JAK2 V617F was measured with quantitative PCR.RESULTS:
The clinical and hematological findings of 27 patients were compatible with diagnosis of MDS/MPN. PDGFRß rearrangement was detected in 4 patients with D-FISH, and 2 of which were confirmed as t(5;12) by chromosome painting. PDGFRα, FGFR1 and JAK2 V617F mutation were not detected in these 4 PDGFRß positive MDS/MPN patients with.CONCLUSIONS:
PDGFRß gene rearrangement may be detected in some MDS/MPN patients. FISH is a convenient and reliable approach to detect PDGFRß gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hibridização in Situ Fluorescente
/
Receptor beta de Fator de Crescimento Derivado de Plaquetas
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Xue Ye Xue Za Zhi
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China