Yox1 links MBF-dependent transcription to completion of DNA synthesis.
EMBO Rep
; 12(1): 84-9, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-21132016
ABSTRACT
When DNA replication is challenged cells activate a DNA synthesis checkpoint, blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M-phase entry, stabilizing stalled replication forks and triggering transcriptional activation of S-phase genes. The molecular basis of this last effect is largely unknown. The Mlu1 binding factor (MBF) complex controls the transcription of S-phase genes. We purified novel interactors of the MBF complex and identified the repressor Yox1. When the DNA synthesis checkpoint is activated, Yox1 is phosphorylated, which abrogates its binding to MBF. MBF-dependent transcription therefore remains active until cells are able to overcome this challenge.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Fatores de Transcrição
/
DNA Fúngico
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Homeodomínio
/
Proteínas de Ciclo Celular
/
Proteínas de Schizosaccharomyces pombe
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
EMBO Rep
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Espanha