Soil and plant contamination with Mycobacterium avium subsp. paratuberculosis after exposure to naturally contaminated mouflon feces.
Curr Microbiol
; 62(5): 1405-10, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-21279514
ABSTRACT
The aim of this study was to demonstrate the persistence of Mycobacterium avium subsp. paratuberculosis (MAP) in soil and colonization of different plant parts after deliberate exposure to mouflon feces naturally contaminated with different amounts of MAP. Samples of aerial parts of plants, their roots, and the soil below the roots were collected after 15 weeks and examined using IS900 real-time quantitative PCR (qPCR) and cultivation. Although the presence of viable MAP cells was not demonstrated, almost all samples were found to be positive using qPCR. MAP IS900 was not only found in the upper green parts, but also in the roots and soil samples (from 1.00 × 10(0) to 6.43 × 10(3)). The level of soil and plant contamination was influenced mainly by moisture, clay content, and the depth from which the samples were collected, rather than by the initial concentration of MAP in the feces at the beginning of the experiment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paratuberculose
/
Plantas
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Doenças dos Ovinos
/
Microbiologia do Solo
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Mycobacterium avium subsp. paratuberculosis
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Carneiro Doméstico
/
Fezes
Limite:
Animals
Idioma:
En
Revista:
Curr Microbiol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
República Tcheca