Effects of exercise training on gingival oxidative stress in obese rats.
Arch Oral Biol
; 56(8): 768-74, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21315319
ABSTRACT
OBJECTIVE:
The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet.DESIGN:
Rats were divided into three groups (n = 14/group) one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress.RESULTS:
The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean ± SD; 413 ± 64) than the control (333 ± 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 ± 0.18) (p < 0.05) and a lower GSH/GSSG ratio (17.0 ± 3.1) (p < 0.05) in gingival tissues, compared to the control (0.55 ± 0.13 for 8-OHdG and 23.6 ± 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 ± 103) (p < 0.001) and gingival 8-OHdG levels (0.69 ± 0.17) (p = 0.012) than those without exercise training (1105 ± 95 for ROM and 0.55 ± 0.13 for 8-OHdG) at 8 weeks.CONCLUSIONS:
Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
/
Estresse Oxidativo
/
Gengiva
/
Obesidade
Limite:
Animals
Idioma:
En
Revista:
Arch Oral Biol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão