Kasahara-variant alkaline phosphatase in a renal cell carcinoma.
Clin Chim Acta
; 89(2): 311-6, 1978 Oct 16.
Article
em En
| MEDLINE
| ID: mdl-213214
ABSTRACT
Electrophoretically Kasahara-variant alkaline phosphatase we found in a renal cell carcinoma tissue. This enzyme electrophoresed more quickly than liver alkaline phosphatase but more slowly than Kasahara isoenzyme. Neuraminidase treatment of the enzyme caused retardation of electrophoretic mobility which was the same as that of neuraminidase-treated Kasahara isoenzyme. The enzymic properties of this variant enzyme such as inhibition by L-phenylalanine, L-homoarginine, L-leucine, EDTA and urea are consistent with those of Kasahara isoenzyme. On Ouchterlony double diffusion, the precipitin lines of Kasahara and Kasahara-variant enzymes produced by antibody to Kasahara isoenzyme fused completely. These facts may mean that Kasahara-variant isoenzyme is different from the Kasahara one in terminal sialic acid content.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatase Alcalina
/
Neoplasias Renais
Limite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
1978
Tipo de documento:
Article