[Preparative isolation and purification of the active components from Viticis Fructus by high-speed counter-current chromatography].
Se Pu
; 28(11): 1043-7, 2010 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-21381420
ABSTRACT
Vitex trifolia L. var. simplicifolia Cham. is widely distributed in Asia, and its fruits are used as a folk medicine for headaches, colds, migraine, eyepain, etc. In order to effectively separate high-purity active components from the seeds of Vitex trifolia L. var. simplicifolia Cham., a high-speed counter-current chromatography (HSCCC) procedure was performed to separate four components from the crude extract of the fruits. A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water (36 3.6 3, v/v/v/ v) was used. Within 230 min, 23 mg of 4-hydroxybenzoic acid, 15 mg of 3,6,7-trimethylquercetagetin, 24 mg of casticin and 5 mg of artemetin were obtained from 250 mg of the crude extract of Viticis Fructus in one-step elution under the conditions of a flow rate of 1.5 mL/min, 800 r/min and the detection wavelength of 254 nm. The purities of the four fractions were 93.1%, 97.3%, 98.7% and 98.5%, respectively. The obtained fractions were analyzed by high performance liquid chromatography (HPLC), and identified by electrospray ionization mass spectrometry (ESI-MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR. The results indicate that HSCCC is a powerful technique for the purification of active components from Viticis Fructus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parabenos
/
Flavonoides
/
Cromatografia Líquida de Alta Pressão
/
Distribuição Contracorrente
/
Vitex
Idioma:
Zh
Revista:
Se Pu
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China