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Quantitative evaluation of microscopic injury with diffusion tensor imaging in a rat model of diffuse axonal injury.
Li, Jia; Li, Xue-Yuan; Feng, Dong-Fu; Gu, Lei.
Afiliação
  • Li J; Department of Neurosurgery, No. 3 People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 280 Mo-He Road, Shanghai 201900, China.
Eur J Neurosci ; 33(5): 933-45, 2011 Mar.
Article em En | MEDLINE | ID: mdl-21385236
ABSTRACT
Diffuse axonal injury (DAI) is the predominant effect of severe traumatic brain injury and contributes significantly to neurological deficits. However, it is difficult to diagnose or characterize non-invasively with conventional imaging. Our study provides significant validation of a visual and statistical diffusion tensor imaging (DTI) technique as compared with pathological and electron microscopic study in a rat DAI model at multiple predilection sites and time points following trauma. Two DTI parameters, fractional anisotropy (FA) and axial diffusivity (AD), were significantly reduced from 12 h to 5 days post-trauma, corresponding to pathological axonal injury. At 7 days post-trauma, FA remained decreased, whereas AD pseudo-normalized and radial diffusivity increased. The temporal alterations in DTI parameters were observed in multiple predilection sites, and the extent of the changes in these parameters correlated significantly with the severity of histologically visualized axonal injury, as assessed by integrated optical density of immunochemically stained injured axons with quantitative stereology. Although anatomical T2-weighted magnetic resonance images showed no abnormal signals in microscopic lesions, we detected and characterized axonal injury directly by DTI at each time point. These results demonstrate that DTI has significant potential as a non-invasive tool with which to quantitatively diagnose and evaluate microstructural injury in the experimental and clinical assessment of DAI. This method can assist in accurate evaluation of the extent of axonal injury, detection of severe predilection foci, determination of approximate time of injury, and monitoring of the pathogenic condition at the early post-injury stage.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões Encefálicas / Lesão Axonal Difusa / Imagem de Tensor de Difusão Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões Encefálicas / Lesão Axonal Difusa / Imagem de Tensor de Difusão Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China