Sphingomyelin synthase overexpression increases cholesterol accumulation and decreases cholesterol secretion in liver cells.
Lipids Health Dis
; 10: 46, 2011 Mar 21.
Article
em En
| MEDLINE
| ID: mdl-21418611
ABSTRACT
BACKGROUND:
Studies have shown that plasma high density lipoprotein cholesterol levels are negatively correlated with the development of atherosclerosis, whereas epidemiological studies have also shown that plasma sphingomyelin level is an independent risk factor for atherosclerosis.METHODS:
To evaluate the relationship between cellular sphingomyelin level and cholesterol metabolism, we created two cell lines that overexpressed sphingomyelin synthase 1 or 2 (SMS1 or SMS2), using the Tet-off expression system.RESULTS:
We found that SMS1 or SMS2 overexpression in Huh7 cells, a human hepatoma cell line, significantly increased the levels of intracellular sphingomyelin, cholesterol, and apolipoprotein A-I and decreased levels of apolipoprotein A-I and cholesterol in the cell culture medium, implying a defect in both processes.CONCLUSIONS:
Our findings indicate that the manipulation of sphingomyelin synthase activity could influence the metabolism of sphingomyelin, cholesterol and apolipoprotein A-I.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colesterol
/
Transferases (Outros Grupos de Fosfato Substituídos)
/
Fígado
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Lipids Health Dis
Assunto da revista:
BIOQUIMICA
/
METABOLISMO
Ano de publicação:
2011
Tipo de documento:
Article