CD36 is involved in lycopene and lutein uptake by adipocytes and adipose tissue cultures.
Mol Nutr Food Res
; 55(4): 578-84, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-21462325
ABSTRACT
SCOPE Carotenoids are mainly stored in adipose tissue. However, nothing is known regarding the uptake of carotenoids by adipocytes. Thus, our study explored the mechanism by which lycopene and lutein, two major human plasma carotenoids, are transported. METHODS AND RESULTS:
CD36 was a putative candidate for this uptake, 3T3-L1 cells were treated with sulfosuccinimidyl oleate, a CD36-specific inhibitor. sulfosuccinimidyl oleate-treated cells showed a significant decrease in both lycopene and lutein uptake as compared to control cells. Their uptake was also decreased by partial inhibition of CD36 expression using siRNA, whereas the overexpression of CD36 in Cos-1 cells increased their uptake. Finally, the effect of CD36 on carotenoid uptake was confirmed ex vivo in cultures of adipose tissue explants from CD36(-/-) mice, which exhibited reduced carotenoid uptake as compared to wild-type mice explants.CONCLUSION:
For the first time, we report the involvement of a transporter, CD36, in carotenoid uptake by adipocytes and adipose tissue.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Luteína
/
Carotenoides
/
Antígenos CD36
/
Tecido Adiposo Branco
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Mol Nutr Food Res
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
França