Inactivation of Ca2(+)-, Na+K(+)-, and H+K(+)-ATPases with a carbodiimide derivative of ATP.
FEBS Lett
; 263(1): 175-7, 1990 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-2158904
ABSTRACT
The gamma-P adduct of ATP with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (ATP-EDC) was synthesized and incubated with the Ca-ATPase of sarcoplasmic reticulum with the result that time-dependent complete loss of the enzyme's activity occurred. The inactivation required calcium and magnesium while ATP had a protective effect. ATP-EDC incubation with the NaK-ATPase and HK-ATPase produced partial (greater than 50%) inactivation, but had no effect on myosin S1, pyruvate kinase and hexokinase, suggesting that this ATP analog is a specific inactivator of the so-called 'P-type' ATPases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retículo Sarcoplasmático
/
Carbodi-Imidas
/
ATPases Transportadoras de Cálcio
/
Adenosina Trifosfatases
/
ATPase Trocadora de Sódio-Potássio
/
Etildimetilaminopropil Carbodi-Imida
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1990
Tipo de documento:
Article