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Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma.
Yao, Min; Yao, Deng-Fu; Bian, Yin-Zhu; Zhang, Chong-Guo; Qiu, Li-Wei; Wu, Wei; Sai, Wen-Li; Yang, Jun-Ling; Zhang, Hai-Jian.
Afiliação
  • Yao M; Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China.
Hepatobiliary Pancreat Dis Int ; 10(3): 289-94, 2011 Jun.
Article em En | MEDLINE | ID: mdl-21669573
ABSTRACT

BACKGROUND:

Hepatocellular carcinoma (HCC) is characterized by a multi-cause, multi-stage and multi-focus process of tumor progression. Its prognosis is poor and early diagnosis is of utmost importance. This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.

METHODS:

A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression. Total RNA was purified and transcribed into cDNA by reverse transcription. Fragments of the GPC-3 gene were amplified by nested RT-PCR, and confirmed by sequencing. GPC-3 was analyzed by immunohistochemistry, Western blotting or ELISA.

RESULTS:

Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm. Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration, atypical hyperplasia (precancerous), and cancer formation, with a progressive increase of liver total RNA and GGT expression. The incidence of liver GPC-3 mRNA and GPC-3, and serum GPC-3 was 100%, 100% and 77.8% in the HCC group, 100%, 100%, and 66.7% in the precancerous group, 83.3%, 83.3%, and 38.9% in the degeneration group, and no expression in the liver or blood of the control group, respectively. There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475, P<0.05) or liver GPC-3 (r=1.0, P<0.001) or serum GPC-3 (r=0.994, P<0.001).

CONCLUSION:

Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Transformação Celular Neoplásica / Carcinoma Hepatocelular / Glipicanas / Neoplasias Hepáticas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals Idioma: En Revista: Hepatobiliary Pancreat Dis Int Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Transformação Celular Neoplásica / Carcinoma Hepatocelular / Glipicanas / Neoplasias Hepáticas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals Idioma: En Revista: Hepatobiliary Pancreat Dis Int Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China