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BiP and multiple DNAJ molecular chaperones in the endoplasmic reticulum are required for efficient simian virus 40 infection.
Goodwin, Edward C; Lipovsky, Alex; Inoue, Takamasa; Magaldi, Thomas G; Edwards, Anne P B; Van Goor, Kristin E Y; Paton, Adrienne W; Paton, James C; Atwood, Walter J; Tsai, Billy; DiMaio, Daniel.
Afiliação
  • Goodwin EC; Department of Genetics, Yale School of Medicine, New Haven, Connecticut, USA.
mBio ; 2(3): e00101-11, 2011.
Article em En | MEDLINE | ID: mdl-21673190
ABSTRACT
Simian virus 40 (SV40) is a nonenveloped DNA virus that traffics through the endoplasmic reticulum (ER) en route to the nucleus, but the mechanisms of capsid disassembly and ER exit are poorly understood. We conducted an unbiased RNA interference screen to identify cellular genes required for SV40 infection. SV40 infection was specifically inhibited by up to 50-fold by knockdown of four different DNAJ molecular cochaperones or by inhibition of BiP, the Hsp70 partner of DNAJB11. These proteins were not required for the initiation of capsid disassembly, but knockdown markedly inhibited SV40 exit from the ER. In addition, BiP formed a complex with SV40 capsids in the ER in a DNAJB11-dependent fashion. These experiments identify five new cellular proteins required for SV40 infection and suggest that the binding of BiP to the capsid is required for ER exit. Further studies of these proteins will provide insight into the molecular mechanisms of polyomavirus infection and ER function.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus 40 dos Símios / Chaperonas Moleculares / Retículo Endoplasmático / Proteínas de Choque Térmico HSP40 / Interações Hospedeiro-Patógeno / Proteínas de Choque Térmico Limite: Animals / Humans Idioma: En Revista: MBio Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus 40 dos Símios / Chaperonas Moleculares / Retículo Endoplasmático / Proteínas de Choque Térmico HSP40 / Interações Hospedeiro-Patógeno / Proteínas de Choque Térmico Limite: Animals / Humans Idioma: En Revista: MBio Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos