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Synthesis, phosphorylation, and nuclear localization of human papillomavirus E7 protein in Schizosaccharomyces pombe.
Tommasino, M; Contorni, M; Scarlato, V; Bugnoli, M; Maundrell, K; Cavalieri, F.
Afiliação
  • Tommasino M; Molecular Virology, Sclavo Research Centre, Siena, Italy.
Gene ; 93(2): 265-70, 1990 Sep 14.
Article em En | MEDLINE | ID: mdl-2172091
The complete E7 protein-encoding open reading frame of human papillomavirus type 16 (HPV-16) was expressed in the fission yeast Schizosaccharomyces pombe, under the control of a cloned yeast promoter. The HPV-16 E7 protein synthesized in S. pombe is a 17-kDa phosphoprotein which is recognized by anti-E7 antibodies (raised in rabbits against E7 fusion protein produced in Escherichia coli). The mobility during sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of native E7 phosphoprotein synthesized in S. pombe is identical to that of the E7 phosphoprotein immunoprecipitated from human CaSki cells. Immunofluorescence staining showed that HPV-16 E7 phosphoprotein is localized in the nuclei of transformed S. pombe. These results indicate that E7 protein synthesized by S. pombe is apparently indistinguishable from HPV-16 E7 protein synthesized in higher eukaryotic cells expressing genes of HPV-16, and also that the phosphorylated, nuclear HPV-16 E7 protein is synthesized in S. pombe in a form compatible with its biological activity.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfoproteínas / Schizosaccharomyces / Proteínas Oncogênicas Virais Limite: Animals / Humans Idioma: En Revista: Gene Ano de publicação: 1990 Tipo de documento: Article País de afiliação: Itália
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfoproteínas / Schizosaccharomyces / Proteínas Oncogênicas Virais Limite: Animals / Humans Idioma: En Revista: Gene Ano de publicação: 1990 Tipo de documento: Article País de afiliação: Itália