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Fine phenotypic and functional characterization of effector cluster of differentiation 8 positive T cells in human patients with primary biliary cirrhosis.
Tsuda, Masanobu; Ambrosini, Yoko M; Zhang, Weici; Yang, Guo-Xiang; Ando, Yugo; Rong, Guanghua; Tsuneyama, Koichi; Sumida, Kosuke; Shimoda, Shinji; Bowlus, Christopher L; Leung, Patrick S C; He, Xiao-Song; Coppel, Ross L; Ansari, Aftab A; Lian, Zhe-Xiong; Gershwin, M Eric.
Afiliação
  • Tsuda M; Division of Rheumatology, Allergy and Clinical Immunology, University of California at Davis, Davis, CA 95616, USA.
Hepatology ; 54(4): 1293-302, 2011 Oct.
Article em En | MEDLINE | ID: mdl-21735469
ABSTRACT
UNLABELLED In primary biliary cirrhosis (PBC), patients develop a multilineage response to a highly restricted peptide of the E2 component of pyruvate dehydrogenase (PDC-E2) involving autoantibody and autoreactive cluster of differentiation (CD)4(+) and CD8(+) T-cell responses. Recent data from murine models have suggested that liver-infiltrating CD8(+) cells play a critical role in biliary destruction in PBC. We hypothesized that chronic antigen stimulation of CD8(+) T cells alters effector memory T cell (T(EM) ) frequency and function similar to that seen with chronic viral infections, including failure to terminally differentiate and relative resistance to apoptosis. We have rigorously phenotyped CD8(+) T-cell subpopulations from 132 subjects, including 76 patients with PBC and 56 controls, and report a higher frequency of T(EM) cells characterized as CD45RO(high) CD57(+) CD8(high), but expressing the gut homing integrin, α4ß7, in peripheral blood mononuclear cells of PBC. These CD8(high) T(EM) cells have reduced expression of Annexin V after TCR stimulation. Consistent with a T(EM) phenotype, CD45RO(high) CD57(+) CD8(high) T cells express higher levels of granzyme A, granzyme B, perforin, CCR5 and α4ß7, and lower levels of CCR7 and CD28 than other CD8(high) T cells. Furthermore, interleukin (IL)-5 produced by CD8(+) CD57(+) T lymphocytes upon in vitro T-cell receptor stimulation are increased in PBC. Histologically, CD8(+) CD57(+) T cells accumulate around the portal area in PBC. Moreover, CD8(+) CD57(+) T cells respond specifically to the major histocompatibility class I epitope of PDC-E2.

CONCLUSION:

In conclusion, our data demonstrate that CD45RO(high) CD57(+) CD8(high) T cells are a subset of terminally differentiated cytotoxic T(EM) cells, which could play a critical role in the progressive destruction of biliary epithelial cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Linfócitos T CD8-Positivos / Cirrose Hepática Biliar Tipo de estudo: Observational_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Hepatology Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Linfócitos T CD8-Positivos / Cirrose Hepática Biliar Tipo de estudo: Observational_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Hepatology Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos