Expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system.
Sci China B
; 33(9): 1070-7, 1990 Sep.
Article
em En
| MEDLINE
| ID: mdl-2173617
ABSTRACT
The expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system was investigated. The human TK- 143 cells were infected with the recombinant vaccinia viruses vTMS-1 or vTLS-1. Cells infected with vTMS-1, which contains the preS2 + S gene, produced preS2 containing middle HBsAg proteins. Similarly, cells produced preS1 containing large HBsAg proteins upon infection with vTLS-1, which carries the preS1 + preS2 + S gene. The expression products could be secreted and form 22 nm particles. They reacted specifically with anti-preS1 and/or anti-preS2 monoclonal antibodies, and exhibited pHSA-receptor (for polymerized human serum albumin) activity. In addition, the major S components of hepatitis B surface antigen were also present in the products expressed by vTMS-1 and vTLS-1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Vaccinia virus
/
Vírus da Hepatite B
/
Genes Virais
/
Antígenos de Superfície da Hepatite B
Limite:
Humans
Idioma:
En
Revista:
Sci China B
Assunto da revista:
BIOLOGIA
/
CIENCIA
/
QUIMICA
Ano de publicação:
1990
Tipo de documento:
Article