A polymorphism-specific "memory" mechanism in the ß(2)-adrenergic receptor.
Sci Signal
; 4(185): ra53, 2011 Aug 09.
Article
em En
| MEDLINE
| ID: mdl-21868359
ABSTRACT
Signaling through G protein (heterotrimeric guanosine triphosphate-binding protein)-coupled receptors is affected by polymorphisms in receptor-encoding genes. Using fluorescence resonance energy transfer, we found that the ß(2)-adrenergic receptor (ß(2)AR) responded to repeated activation with altered activation kinetics. Polymorphic variants of the ß(2)AR displayed divergent changes of ß(2)AR activation kinetics that closely mimicked their different efficacies to generate cyclic adenosine 3',5'-monophosphate. More efficacious variants became faster in their activation kinetics, whereas less efficacious variants became slower, compared to their initial activation. These differences depended on phosphorylation of the receptor by G protein-coupled receptor kinases. Our findings suggest an intrinsic, polymorphism-specific property of the ß(2)AR that alters activation kinetics upon continued stimulation and that may account for individual drug responses.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Transdução de Sinais
/
Receptores Adrenérgicos beta 2
/
AMP Cíclico
Limite:
Humans
Idioma:
En
Revista:
Sci Signal
Assunto da revista:
CIENCIA
/
FISIOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Alemanha