Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A.
FEBS Lett
; 586(4): 373-7, 2012 Feb 17.
Article
em En
| MEDLINE
| ID: mdl-22245677
ABSTRACT
A previously uncharacterized Arabidopsis lecithincholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine>phosphatidic acid>phosphatidylethanolamine>phosphatidylglycerol>phosphatidylserine and has an acyl chain specificity for oleoyl>linoleoyl>ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipases A
/
Genes de Plantas
/
Arabidopsis
/
Proteínas de Arabidopsis
/
Fosfatidilcolina-Esterol O-Aciltransferase
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Canadá