The conclusiveness of less-invasive imaging techniques (computer tomography, X-ray) with regard to their identification of bone diseases in a primate model (Callithrix jacchus).
J Med Primatol
; 41(2): 130-7, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22300635
ABSTRACT
BACKGROUND:
Although common marmosets seem to be appropriate animal models to examine bone diseases, no data about the conclusiveness of less-invasive techniques are available. Therefore, the aim was to combine different techniques to analyse changes in bone metabolism of common marmosets with bone diseases.METHODS:
Five monkeys were examined by X-ray, computer tomography (CT), histology and immunohistochemistry (IHC).RESULTS:
Monkeys with lowest bone mineral density (BMD) showed increased bone marrow, decreased cancellous bone and decreased contrast in X-ray. Highest alkaline phosphatase (AP)-levels were detected in bones with low elastic modulus. Expression of osteopontin (OPN), osteocalcin (OC) and runt-related transcriptions factor 2 (RUNX 2) was detected in bones with high modulus. No expression was present in bones with lower modulus. Collagen type I and V were found in every bone.CONCLUSIONS:
In conclusion, CT, X-ray and AP are useful techniques to detect bone diseases in common marmosets. These observations could be confirmed by IHC.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osso e Ossos
/
Doenças Ósseas
/
Callithrix
/
Tomografia Computadorizada por Raios X
/
Modelos Animais
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Med Primatol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Alemanha