MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration.
Proteome Sci
; 10(1): 7, 2012 Feb 04.
Article
em En
| MEDLINE
| ID: mdl-22304756
ABSTRACT
Naturally occurring native peptides provide important information about physiological states of an organism and its changes in disease conditions but protocols and methods for assessing their abundance are not well-developed. In this paper, we describe a simple procedure for the quantification of non-tryptic peptides in body fluids. The workflow includes an enrichment step followed by two-dimensional fractionation of native peptides and MS/MS data management facilitating the design and validation of LC- MRM MS assays. The added value of the workflow is demonstrated in the development of a triplex LC-MRM MS assay used for quantification of peptides potentially associated with the progression of liver disease to hepatocellular carcinoma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Proteome Sci
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos