Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti.
J Hered
; 103(2): 303-7, 2012.
Article
em En
| MEDLINE
| ID: mdl-22308303
ABSTRACT
An endogenous meiotic driver in the dengue and yellow fever vector mosquito Aedes aegypti can cause highly male-biased sex ratio distortion in crosses from suitable genetic backgrounds. We previously selected a strain that carries a strong meiotic drive gene (D) linked with the male-determining allele (M) on chromosome 1 in A. aegypti. Here, we performed segregation analysis of the M(D) locus among backcross (BC(1)) progeny from a driver male and drive-sensitive females. Assessment of sex ratios among BC(2) progeny showed â¼5.2% recombination between the M(D) locus and the sex determination locus. Multipoint linkage mapping across this region revealed consistent marker orders and recombination frequencies with the existing reference linkage map and placed the M(D) locus within a 6.5-cm interval defined by the LF159 locus and microsatellite marker 446GAA, which should facilitate future positional cloning efforts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Razão de Masculinidade
/
Genes de Insetos
/
Aedes
/
Meiose
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Hered
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos