[Sequence-based typing of 82 strains of serotype I Legionella pneumophila isolated from 9 provinces in China].
Zhonghua Yu Fang Yi Xue Za Zhi
; 45(10): 890-4, 2011 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-22321587
ABSTRACT
OBJECTIVE:
To analyze the characteristics of Sequence-based Typing (SBT) of the Serotype 1 Legionella pneumophila (Lp1) isolated from environmental water in China, and then create a preliminary database.METHODS:
A total of 82 strains of Lp1 isolated from environmental water in 9 provinces of China between 2005 and 2008 were genotyped by SBT method and Pulsed-field Gel Electrophoresis (PFGE) method. The results of the two different typing methods were then compared by cluster analysis, adopting BioNumerics version 5.1 software.RESULTS:
By SBT method, the 82 strains of Lp1 were divided into 22 ST types, of which 17 new types and one new allele was discovered. The dominant type was ST-1 type, found in 8 provinces, accounting for 46.3% (38/82). ST-1, ST-150, ST-154, ST-159, ST-160 and ST-630 types were found in more than 2 isolated-sites; while more than 2 different ST types were found in 5 isolated-sites, as site B4, B5, B6, S3 and S8. In cluster analysis, 15 ST types were grouped into three complexes (ST-1 complex, ST-154 complex and ST-149 complex); and the other 7 ST types were not assigned complex. By PFGE method, 46 banding patterns were observed. As a result of the combination of the two methods, the 82 isolates strains could be divided into 54 molecular types, which showed a reliable accordance in the cluster analysis between the two methods.CONCLUSION:
The SBT of the Lp1 in environmental water in China was unique. From the study, a preliminary SBT database was set up.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sorotipagem
/
Legionella pneumophila
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Yu Fang Yi Xue Za Zhi
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China