Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle X-ray scattering.
Methods Mol Biol
; 849: 137-55, 2012.
Article
em En
| MEDLINE
| ID: mdl-22528088
ABSTRACT
Structural investigation of the species present during protein fibrillation is of tremendous importance, yet complicated by the equilibrium between species of very different sizes and life-times. Small-angle X-ray scattering may be applied to solve this problem, providing both information about the process (number of species present and volume fractions of individual species) and low-resolution three-dimensional shape reconstructions of individual species. Here, we describe in detail the challenges associated with the approach, exemplified using data from fibrillating insulin or α-synuclein samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Difração de Raios X
/
Alfa-Sinucleína
/
Espalhamento a Baixo Ângulo
/
Multimerização Proteica
/
Amiloide
/
Insulina
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Dinamarca