Development of polymorphic microsatellite markers for Cymbidium goeringii (Orchidaceae).

ABSTRACT

PREMISE OF THE STUDY Microsatellite markers were developed in Cymbidium goeringii to investigate its genetic diversity and population genetic structure. METHODS AND RESULTS: From a microsatellite-enriched genomic library, 21 novel polymorphic microsatellites were isolated. The polymorphic patterns were verified in four populations (East Korea, West Korea, China, and Japan). The number of alleles per locus ranged from 11 to 29 with a mean of 20.29. The observed and expected heterozygosities varied from 0.272 to 0.799 and from 0.461 to 0.911, respectively. CONCLUSIONS: These microsatellite markers will be useful tools for understanding genetic variation and population ecogenetic structure in C. goeringii.