2b-RAD: a simple and flexible method for genome-wide genotyping.
Nat Methods
; 9(8): 808-10, 2012 May 20.
Article
em En
| MEDLINE
| ID: mdl-22609625
ABSTRACT
We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Desoxirribonucleases de Sítio Específico do Tipo II
/
Genoma
/
Técnicas de Genotipagem
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos