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Amniotic fluid stem cells restore the muscle cell niche in a HSA-Cre, Smn(F7/F7) mouse model.
Stem Cells ; 30(8): 1675-84, 2012 Aug.
Article em En | MEDLINE | ID: mdl-22644669
ABSTRACT
Mutations in the survival of motor neuron gene (SMN1) are responsible for spinal muscular atrophy, a fatal neuromuscular disorder. Mice carrying a homozygous deletion of Smn exon 7 directed to skeletal muscle (HSA-Cre, Smn(F7/F7) mice) present clinical features of human muscular dystrophies for which new therapeutic approaches are highly warranted. Herein we demonstrate that tail vein transplantation of mouse amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. Second, after cardiotoxin injury of the Tibialis Anterior, only AFS-transplanted mice efficiently regenerate. Most importantly, secondary transplants of satellite cells (SCs) derived from treated mice show that AFS cells integrate into the muscle stem cell compartment and have long-term muscle regeneration capacity indistinguishable from that of wild-type-derived SC. This is the first study demonstrating the functional and stable integration of AFS cells into the skeletal muscle, highlighting their value as cell source for the treatment of muscular dystrophies.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Atrofia Muscular Espinal / Músculo Esquelético / Transplante de Células-Tronco / Nicho de Células-Tronco / Líquido Amniótico Limite: Animals Idioma: En Revista: Stem Cells Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Atrofia Muscular Espinal / Músculo Esquelético / Transplante de Células-Tronco / Nicho de Células-Tronco / Líquido Amniótico Limite: Animals Idioma: En Revista: Stem Cells Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Itália