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A BioBrick compatible strategy for genetic modification of plants.
Boyle, Patrick M; Burrill, Devin R; Inniss, Mara C; Agapakis, Christina M; Deardon, Aaron; DeWerd, Jonathan G; Gedeon, Michael A; Quinn, Jacqueline Y; Paull, Morgan L; Raman, Anugraha M; Theilmann, Mark R; Wang, Lu; Winn, Julia C; Medvedik, Oliver; Schellenberg, Kurt; Haynes, Karmella A; Viel, Alain; Brenner, Tamara J; Church, George M; Shah, Jagesh V; Silver, Pamela A.
Afiliação
  • Boyle PM; Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
  • Burrill DR; Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
  • Inniss MC; Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
  • Agapakis CM; Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
  • Deardon A; Current Address: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
  • DeWerd JG; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Gedeon MA; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Quinn JY; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Paull ML; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Raman AM; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Theilmann MR; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Wang L; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Winn JC; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Medvedik O; Harvard College, Harvard University, Cambridge, MA, 02138, USA.
  • Schellenberg K; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, 02138, USA.
  • Haynes KA; The Arnold Arboretum of Harvard University, Boston, MA, 02131, USA.
  • Viel A; Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
  • Brenner TJ; Current Address: School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, 85287, USA.
  • Church GM; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, 02138, USA.
  • Shah JV; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, 02138, USA.
  • Silver PA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
J Biol Eng ; 6(1): 8, 2012 Jun 20.
Article em En | MEDLINE | ID: mdl-22716313
ABSTRACT

BACKGROUND:

Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process.

RESULTS:

This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (http//www.partsregistry.org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin.

CONCLUSIONS:

Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Biol Eng Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Biol Eng Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Estados Unidos