Comparison of performance of serum and plasma in panbio dengue and Japanese encephalitis virus enzyme-linked immunosorbent assays.
Am J Trop Med Hyg
; 87(3): 573-575, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22826488
ABSTRACT
We examined the comparative performance of serum and plasma (in dipotassium EDTA) in Panbio Dengue enzyme-linked immunosorbent assays (ELISAs) for detection of non-structural protein 1 (NS1), IgM, and IgG, and a dengue/Japanese encephalitis virus (JEV) combination IgM ELISA in a prospective series of 201 patients with suspected dengue in Laos. Paired comparisons of medians from serum and plasma samples were not significantly different for Dengue IgM, and NS1 which had the highest number of discordant pairs (both 2%; P = 0.13 and P = 0.25, respectively). Comparison of qualitative final diagnostic interpretations for serum and plasma samples were not significantly different only 1.5% (3 of 201 for Dengue/JEV IgM and Dengue IgG) and 2.0% (4 of 201; IgM and NS1) showed discordant pairs. These results demonstrate that plasma containing EDTA is suitable for use in these ELISAs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Ensaio de Imunoadsorção Enzimática
/
Encefalite Japonesa
/
Dengue
/
Soro
Tipo de estudo:
Observational_studies
/
Qualitative_research
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2012
Tipo de documento:
Article