Isolation of satellite cells from single muscle fibers from young, aged, or dystrophic muscles.
Methods Mol Biol
; 916: 3-14, 2012.
Article
em En
| MEDLINE
| ID: mdl-22914928
ABSTRACT
Skeletal muscle contains an identified resident stem cell population called the satellite cells. This cell is responsible for the majority of the postnatal growth and regenerative potential of skeletal muscle. Other cells do contribute to skeletal muscle regeneration and in cultures of minced whole muscle these cells are cultured along with the satellite cells and it is impossible to dissect out their contribution compared to the satellite cells. Therefore, a method to culture pure satellite cells has been developed to study the signaling pathways that control their proliferation and differentiation. In our studies into the role of the resident myogenic stem cells in regeneration, myopathic conditions, and aging, we have optimized the established techniques that already exist to isolate pure satellite cell cultures from single muscle fibers. We have successfully isolated satellite cells from young adults through to 24-month-old muscles and obtained populations of cells that we are studying for the signaling events that regulate their proliferative potential.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Envelhecimento
/
Separação Celular
/
Fibras Musculares Esqueléticas
/
Células Satélites de Músculo Esquelético
/
Distrofias Musculares
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Reino Unido