Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.
Nat Methods
; 9(10): 973-5, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22941364
ABSTRACT
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endonucleases
/
Quebras de DNA de Cadeia Dupla
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos