Establishment of a new method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 34(4): 343-7, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22954115
ABSTRACT
OBJECTIVE:
To establish a new culture method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells.METHODS:
Mouse embryos at day 12.5 were used and embryonic pancreata were isolated. The isolated embryonic pancreata were cultured on the filter for 7 days, which floated in the dish containing medium. During culture, the expression of pancreas duodenum homeobox-1 (PDX-1), a pancreatic stem cell marker, was examined at day 1. The expression of neurogenin 3 (Ngn3), a pancreatic progenitor cell marker, was examined at day 3. The expressions of endocrine and exocrine markers, insulin, glucagon, and carboxypeptidase (CPA) were examined at day 7 by immunohistochemistry. The kinetics of pancreatic marker expression during culture was assayed by real-time PCR.RESULTS:
Many pancreatic stem cells still existed in embryonic pancreata cultured for 1 day; meanwhile, these pancreatic stem cells proliferated in high rate. A large amount of pancreatic progenitor cells were found in embryonic pancreata cultured for 3 days.Pancreatic stem/progenitor cells differentiated into mature endocrine and exocrine cells in embryonic pancreata after having been cultured for 7 days. Furthermore, the expression pattern of pancreatic marker is consistent with that in vivo.CONCLUSION:
We successfully established a new culture method, with which embryonic pancreatic cells can efficiently differentiate into mature endocrine cell.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
Diferenciação Celular
/
Técnicas de Cultura de Células
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Embrião de Mamíferos
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Células Endócrinas
Limite:
Animals
Idioma:
En
Revista:
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China